› 4a36

X-ray diffraction
3.7Å resolution

Structure of duck RIG-I helicase domain bound to 19-mer dsRNA and ATP transition state analogue

Released:
DOI: 10.2210/pdb4a36/pdb
PDB entry 4a36 coloured by chain, front view.
PDB entry 4a36 coloured by chain, side view.
PDB entry 4a36 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the activation of innate immune pattern-recognition receptor RIG-I by viral RNA.
Kowalinski E, Lunardi T, McCarthy AA, Louber J, Brunel J, Grigorov B, Gerlier D, Cusack S
Cell 147 423-35 (2011)
PMID: 22000019

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112548 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 556 amino acids
Theoretical weight: 63.33 KDa
Source organism: Anas platyrhynchos
Expression system: Trichoplusia ni
UniProt:
  • Canonical: D3TI84 (Residues: 242-794; Coverage: 59%)
Gene name: RIG-I
Sequence domains:
Structure domains:
5'-R(*GP*CP*AP*UP*GP*CP*GP*AP*CP*CP*UP*CP*UP*GP *UP*UP*UP*GP*A)-3' Chains: R, T
Molecule details ›
Chains: R, T
Length: 19 nucleotides
Theoretical weight: 6.03 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-R(*UP*CP*AP*AP*AP*CP*AP*GP*AP*GP*GP*UP*CP*GP *CP*AP*UP*GP*C)-3' Chains: S, U
Molecule details ›
Chains: S, U
Length: 19 nucleotides
Theoretical weight: 6.1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand AF3 2 x AF3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41
Unit cell:
a: 129.27Å b: 129.27Å c: 106.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.263
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

164 review citations

Innate immune sensing and signaling of cytosolic nucleic acids.
Wu et al. (2014)
163 more

20 mentions without citation

Molecular mechanisms of RNA interference.
Wilson et al. (2013)
19 more