› 43c9

X-ray diffraction
2.2Å resolution

CRYSTALLOGRAPHIC STRUCTURE OF THE ESTEROLYTIC AND AMIDOLYTIC 43C9 ANTIBODY

Released:
DOI: 10.2210/pdb43c9/pdb
PDB entry 43c9 coloured by chain, front view.
PDB entry 43c9 coloured by chain, side view.
PDB entry 43c9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for amide hydrolysis catalyzed by the 43C9 antibody.
Thayer MM, Olender EH, Arvai AS, Koike CK, Canestrelli IL, Stewart JD, Benkovic SJ, Getzoff ED, Roberts VA
J Mol Biol 291 329-45 (1999)
PMID: 10438624

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208529 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PROTEIN (IMMUNOGLOBULIN (LIGHT CHAIN)) Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 113 amino acids
Theoretical weight: 12.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig heavy chain V region PJ14 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 118 amino acids
Theoretical weight: 12.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01820 (Residues: 19-114; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 104.13Å b: 112.66Å c: 245.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.265
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Computational enzyme design.
Kiss et al. (2013)
4 more

8 mentions without citation

Probability-based protein secondary structure identification using combined NMR chemical-shift data.
Wang et al. (2002)
7 more