› 3udw

X-ray diffraction
2.9Å resolution

Crystal structure of the immunoreceptor TIGIT in complex with Poliovirus receptor (PVR/CD155/necl-5) D1 domain

Released:
DOI: 10.2210/pdb3udw/pdb
PDB entry 3udw coloured by chain, front view.
PDB entry 3udw coloured by chain, side view.
PDB entry 3udw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering.
Stengel KF, Harden-Bowles K, Yu X, Rouge L, Yin J, Comps-Agrar L, Wiesmann C, Bazan JF, Eaton DL, Grogan JL
Proc Natl Acad Sci U S A 109 5399-404 (2012)
PMID: 22421438

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147252 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T-cell immunoreceptor with Ig and ITIM domains Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 11.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q495A1 (Residues: 20-128; Coverage: 48%)
Gene names: TIGIT, VSIG9, VSTM3
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Poliovirus receptor Chains: C, D
Molecule details ›
Chains: C, D
Length: 118 amino acids
Theoretical weight: 13.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15151 (Residues: 28-145; Coverage: 30%)
Gene names: PVR, PVS
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3221
Unit cell:
a: 95.606Å b: 95.606Å c: 149.614Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.251 0.287
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

38 review citations

Lag-3, Tim-3, and TIGIT: Co-inhibitory Receptors with Specialized Functions in Immune Regulation.
Anderson et al. (2016)
37 more

17 mentions without citation

Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering.
Stengel et al. (2012)
16 more