› 3u1l

X-ray diffraction
1.64Å resolution

Structure of the mRNA splicing complex component Cwc2

Released:
DOI: 10.2210/pdb3u1l/pdb
PDB entry 3u1l coloured by chain, front view.
PDB entry 3u1l coloured by chain, side view.
PDB entry 3u1l coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition.
Lu P, Lu G, Yan C, Wang L, Li W, Yin P
Biochem J 441 591-7 (2012)
PMID: 21957909

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing factor CWC2 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 27.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12046 (Residues: 1-240; Coverage: 71%)
Gene names: CWC2, D1041, NTC40, SLC3, YDL209C
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 44.851Å b: 54.367Å c: 111.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.189
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
1 more

4 mentions without citation

Cryo-EM structure of the spliceosome immediately after branching.
Galej et al. (2016)
3 more