› 3ohp

X-ray diffraction
2.04Å resolution

Crystal structure of HGPRT from Vibrio cholerae

Released:
DOI: 10.2210/pdb3ohp/pdb
PDB entry 3ohp coloured by chain, front view.
PDB entry 3ohp coloured by chain, side view.
PDB entry 3ohp coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Kim J, Ramagopal UA, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 177 amino acids
Theoretical weight: 20.14 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KUD7 (Residues: 1-177; Coverage: 100%)
Gene name: VC_0585
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 50.511Å b: 145.213Å c: 51.611Å
α: 90° β: 94.76° γ: 90°
R-values:
R R work R free
0.182 0.18 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links