› 3n1g

X-ray diffraction
1.9Å resolution

Crystal structure of DhhN bound to BOCFn3

Released:
DOI: 10.2210/pdb3n1g/pdb
PDB entry 3n1g coloured by chain, front view.
PDB entry 3n1g coloured by chain, side view.
PDB entry 3n1g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.
Kavran JM, Ward MD, Oladosu OO, Mulepati S, Leahy DJ
J Biol Chem 285 24584-90 (2010)
PMID: 20519495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128900 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Desert hedgehog protein N-product Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43323 (Residues: 24-189; Coverage: 44%)
Gene name: DHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2
Brother of CDO Chains: C, D
Molecule details ›
Chains: C, D
Length: 111 amino acids
Theoretical weight: 12.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BWV1 (Residues: 710-817; Coverage: 10%)
Gene names: BOC, UNQ604/PRO1190
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand ZN 2 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 87.21Å b: 92.985Å c: 74.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The Hedgehog signal transduction network.
Robbins et al. (2012)
6 more

4 mentions without citation

All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner.
Kavran et al. (2010)
3 more