› 3e77

X-ray diffraction
2.5Å resolution

Human phosphoserine aminotransferase in complex with PLP

Released:
DOI: 10.2210/pdb3e77/pdb
PDB entry 3e77 coloured by chain, front view.
PDB entry 3e77 coloured by chain, side view.
PDB entry 3e77 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Lehtio L, Karlberg T, Andersson J, Arrowsmith CH, Berglund H, Bountra C, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Kotenyova T, Moche M, Nilsson ME, Nordlund P, Nyman T, Olesen K, Persson C, Sagemark J, Thorsell SG, Tresaugues L, Van Den Berg S, Welin M, Wikstrom M, Wisniewska M, Weigelt J, Schueler H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 377 amino acids
Theoretical weight: 41.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y617 (Residues: 17-370; Coverage: 96%)
Gene names: PSA, PSAT1
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 171Å b: 171Å c: 103.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.244
Expression system: Escherichia coli

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