› 3bki

X-ray diffraction
1.87Å resolution

Crystal Structure of the GluR2 ligand binding core (S1S2J) in complex with FQX at 1.87 Angstroms

Released:
DOI: 10.2210/pdb3bki/pdb
PDB entry 3bki coloured by chain, front view.
PDB entry 3bki coloured by chain, side view.
PDB entry 3bki coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
6-Azido-7-nitro-1,4-dihydroquinoxaline-2,3-dione (ANQX) forms an irreversible bond to the active site of the GluR2 AMPA receptor.
Cruz LA, Est��banez-Perpi���� E, Pfaff S, Borngraeber S, Bao N, Blethrow J, Fletterick RJ, England PM
J. Med. Chem. 51 5856-60 (2008)
PMID: 18754610

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: B, C, D, P
Molecule details ›
Chains: B, C, D, P
Length: 263 amino acids
Theoretical weight: 29.22 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand FQX 4 x FQX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 62.34Å b: 92.26Å c: 195.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.221
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure and mechanism of glutamate receptor ion channel assembly, activation and modulation.
Mayer ML. (2011)
4 more

1 mention without citation

6-Azido-7-nitro-1,4-dihydroquinoxaline-2,3-dione (ANQX) forms an irreversible bond to the active site of the GluR2 AMPA receptor.
Cruz et al. (2008)