› 3zyd

X-ray diffraction
1.7Å resolution

Crystal structure of 3C protease of coxsackievirus B3

Released:
DOI: 10.2210/pdb3zyd/pdb
PDB entry 3zyd coloured by chain, front view.
PDB entry 3zyd coloured by chain, side view.
PDB entry 3zyd coloured by chain, top view.
Source organism: Coxsackievirus B3
Entry authors: Tan J, Anand K, Mesters JR, Hilgenfeld R

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C PROTEINASE Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.43 KDa
Source organism: Coxsackievirus B3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5UEA2 (Residues: 1541-1723; Coverage: 8%)
Sequence domains: 3C cysteine protease (picornain 3C)
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 76.85Å b: 64.38Å c: 39.36Å
α: 90° β: 116.41° γ: 90°
R-values:
R R work R free
0.194 0.192 0.225
Expression system: Escherichia coli BL21(DE3)

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