› 3zwv

X-ray diffraction
2.3Å resolution

Crystal structure of ADP-ribosyl cyclase complexed with ara-2'F-ADP- ribose at 2.3 angstrom

Released:
DOI: 10.2210/pdb3zwv/pdb
PDB entry 3zwv coloured by chain, front view.
PDB entry 3zwv coloured by chain, side view.
PDB entry 3zwv coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Structural studies of intermediates along the cyclization pathway of Aplysia ADP-ribosyl cyclase.
Kotaka M, Graeff R, Chen Z, Zhang LH, Lee HC, Hao Q
J Mol Biol 415 514-26 (2012)
PMID: 22138343

Function and Biology Details

Reaction catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 260 amino acids
Theoretical weight: 29.72 KDa
Source organism: Aplysia californica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29241 (Residues: 25-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AVU 8 x AVU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 61.146Å b: 76.481Å c: 141.366Å
α: 88.09° β: 90.88° γ: 91.02°
R-values:
R R work R free
0.218 0.215 0.277
Expression system: Komagataella pastoris

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Citations

1 review citation

Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate (NAADP) as messengers for calcium mobilization.
Lee HC. (2012)
3 other articles