› 3zwp

X-ray diffraction
2.11Å resolution

Crystal structure of ADP ribosyl cyclase complexed with ara-2'F-ADP- ribose at 2.1 angstrom

Released:
DOI: 10.2210/pdb3zwp/pdb
PDB entry 3zwp coloured by chain, front view.
PDB entry 3zwp coloured by chain, side view.
PDB entry 3zwp coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Structural studies of intermediates along the cyclization pathway of Aplysia ADP-ribosyl cyclase.
Kotaka M, Graeff R, Chen Z, Zhang LH, Lee HC, Hao Q
J Mol Biol 415 514-26 (2012)
PMID: 22138343

Function and Biology Details

Reaction catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 260 amino acids
Theoretical weight: 29.72 KDa
Source organism: Aplysia californica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29241 (Residues: 25-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AVU 8 x AVU
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 61.034Å b: 77.61Å c: 140.221Å
α: 87.91° β: 89.24° γ: 88.16°
R-values:
R R work R free
0.188 0.185 0.241
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate (NAADP) as messengers for calcium mobilization.
Lee HC. (2012)
2 other articles

1 mention without citation

Porcine CD38 exhibits prominent secondary NAD(+) cyclase activity.
Ting et al. (2016)