› 3zs9

X-ray diffraction
2.1Å resolution

S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment

Released:
DOI: 10.2210/pdb3zs9/pdb
PDB entry 3zs9 coloured by chain, front view.
PDB entry 3zs9 coloured by chain, side view.
PDB entry 3zs9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The mechanism of membrane-associated steps in tail-anchored protein insertion.
Mariappan M, Mateja A, Dobosz M, Bove E, Hegde RS, Keenan RJ
Nature 477 61-6 (2011)
PMID: 21866104

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154015 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 39.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Golgi to ER traffic protein 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 38 amino acids
Theoretical weight: 4.34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40056 (Residues: 1-38; Coverage: 13%)
Gene names: GET2, HUR2, RMD7, YER083C

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 52.558Å b: 77.317Å c: 165.831Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Maintaining a Healthy Proteome during Oxidative Stress.
Reichmann et al. (2018)
26 more

8 mentions without citation

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
7 more