› 3znp

X-ray diffraction
2.4Å resolution

IN VITRO AND IN VIVO INHIBITION OF HUMAN D-AMINO ACID OXIDASE: REGULATION OF D-SERINE CONCENTRATION IN THE BRAIN

Released:
DOI: 10.2210/pdb3znp/pdb
PDB entry 3znp coloured by chain, front view.
PDB entry 3znp coloured by chain, side view.
PDB entry 3znp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural, kinetic, and pharmacodynamic mechanisms of D-amino acid oxidase inhibition by small molecules.
Hopkins SC, Heffernan ML, Saraswat LD, Bowen CA, Melnick L, Hardy LW, Orsini MA, Allen MS, Koch P, Spear KL, Foglesong RJ, Soukri M, Chytil M, Fang QK, Jones SW, Varney MA, Panatier A, Oliet SH, Pollegioni L, Piubelli L, Molla G, Nardini M, Large TH
J Med Chem 56 3710-24 (2013)
PMID: 23631755

Function and Biology Details

Reaction catalysed: 
A D-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-amino-acid oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14920 (Residues: 1-347; Coverage: 100%)
Gene names: DAMOX, DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand SE2 1 x SE2
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS
Spacegroup: P3221
Unit cell:
a: 84.069Å b: 84.069Å c: 189.163Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.232 0.307
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Mouse d-Amino-Acid Oxidase: Distribution and Physiological Substrates.
Koga et al. (2017)
2 more

3 mentions without citation

Competitive Inhibitors Unveil Structure/Function Relationships in Human D-Amino Acid Oxidase.
Molla G. (2017)
2 more