› 3zmf

X-ray diffraction
1.85Å resolution

Salmonella enterica SadA 303-358 fused to GCN4 adaptors (SadAK2)

Released:
DOI: 10.2210/pdb3zmf/pdb
PDB entry 3zmf coloured by chain, front view.
PDB entry 3zmf coloured by chain, side view.
PDB entry 3zmf coloured by chain, top view.
Source organisms:
Primary publication:
A new expression system for protein crystallization using trimeric coiled-coil adaptors.
Hernandez Alvarez B, Hartmann MD, Albrecht R, Lupas AN, Zeth K, Linke D
Protein Eng Des Sel 21 11-8 (2008)
PMID: 18093992

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autotransporter adhesin SadA; General control transcription factor GCN4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 113 amino acids
Theoretical weight: 12.48 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZL64 (Residues: 294-358; Coverage: 5%)
  • Canonical: P03069 (Residues: 250-278; Coverage: 10%)
Gene names: AAS101, AAS3, ARG9, GCN4, STM3691, YEL009C, sadA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3
Unit cell:
a: 46.59Å b: 46.59Å c: 128.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.221
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane.
Leo et al. (2012)
2 more