› 3zhk

X-ray diffraction
1.96Å resolution

The crystal structure of single domain antibody 2x1 scaffold

Released:
DOI: 10.2210/pdb3zhk/pdb
PDB entry 3zhk coloured by chain, front view.
PDB entry 3zhk coloured by chain, side view.
PDB entry 3zhk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Directed evolution of human heavy chain variable domain (VH) using in vivo protein fitness filter.
Kim DS, Song HN, Nam HJ, Kim SG, Park YS, Park JC, Woo EJ, Lim HK
PLoS One 9 e98178 (2014)
PMID: 24892548

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin heavy variable 3-23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01764 (Residues: 20-117; Coverage: 100%)
Gene name: IGHV3-23
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 104.415Å b: 55.701Å c: 74.539Å
α: 90° β: 126.44° γ: 90°
R-values:
R R work R free
0.255 0.253 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Specific in vivo knockdown of protein function by intrabodies.
Marschall et al. (2015)
3 more

2 mentions without citation

Directed evolution of human heavy chain variable domain (VH) using in vivo protein fitness filter.
Kim et al. (2014)
1 more