› 3zga

X-ray diffraction
2Å resolution

Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form

Released:
DOI: 10.2210/pdb3zga/pdb
PDB entry 3zga coloured by chain, front view.
PDB entry 3zga coloured by chain, side view.
PDB entry 3zga coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Crystal structures of bifunctional penicillin-binding protein 4 from Listeria monocytogenes.
Jeong JH, Kim YS, Rojviriya C, Ha SC, Kang BS, Kim YG
Antimicrob Agents Chemother 57 3507-12 (2013)
PMID: 23669378

Function and Biology Details

Reaction catalysed: 
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186949 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
peptidoglycan glycosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 47 amino acids
Theoretical weight: 5.05 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y547 (Residues: 73-119; Coverage: 7%)
Gene name: lmo2229
Structure domains: Rhinovirus 14, subunit 4
peptidoglycan glycosyltransferase Chain: B
Molecule details ›
Chain: B
Length: 537 amino acids
Theoretical weight: 58.1 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y547 (Residues: 178-714; Coverage: 75%)
Gene name: lmo2229
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand CB9 1 x CB9
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21212
Unit cell:
a: 84.813Å b: 127.777Å c: 54.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
1 more

1 mention without citation

Crystal structures of bifunctional penicillin-binding protein 4 from Listeria monocytogenes.
Jeong et al. (2013)