3zef

X-ray diffraction
3.1Å resolution

Crystal structure of Prp8:Aar2 complex: second crystal form at 3.1 Angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-152275 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
A1 cistron-splicing factor AAR2 Chains: A, D
Molecule details ›
Chains: A, D
Length: 355 amino acids
Theoretical weight: 41.73 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32357 (Residues: 1-355; Coverage: 100%)
Gene names: AAR2, YBL06.06, YBL0611, YBL074C
Sequence domains:
Structure domains:
Pre-mRNA-splicing factor 8 Chains: B, E
Molecule details ›
Chains: B, E
Length: 1531 amino acids
Theoretical weight: 176.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33334 (Residues: 885-2413; Coverage: 63%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 125.42Å b: 177.841Å c: 216.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.267
Expression system: Saccharomyces cerevisiae