› 3x3x

X-ray diffraction
1.57Å resolution

Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine

Released:
DOI: 10.2210/pdb3x3x/pdb
PDB entry 3x3x coloured by chain, front view.
PDB entry 3x3x coloured by chain, side view.
PDB entry 3x3x coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Probing the Catalytic Mechanism of Copper Amine Oxidase from Arthrobacter globiformis with Halide Ions.
Murakawa T, Hamaguchi A, Nakanishi S, Kataoka M, Nakai T, Kawano Y, Yamaguchi H, Hayashi H, Tanizawa K, Okajima T
J Biol Chem 290 23094-109 (2015)
PMID: 26269595

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 620 amino acids
Theoretical weight: 68.91 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46881 (Residues: 9-628; Coverage: 97%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand NA 3 x NA
Ligand PEL 2 x PEL
Ligand GOL 7 x GOL
1 modified residue:
Ligand TYQ 2 x TYQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 191.658Å b: 62.89Å c: 158.006Å
α: 90° β: 117.48° γ: 90°
R-values:
R R work R free
0.216 0.215 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ceruloplasmin and other copper binding components of blood plasma and their functions: an update.
Linder MC. (2016)
1 more

2 mentions without citation

Probing the Catalytic Mechanism of Copper Amine Oxidase from Arthrobacter globiformis with Halide Ions.
Murakawa et al. (2015)
1 more