› 3x3n

X-ray diffraction
2Å resolution

Crystal structure of EccB1 of Mycobacterium tuberculosis in spacegroup P21

Released:
DOI: 10.2210/pdb3x3n/pdb
PDB entry 3x3n coloured by chain, front view.
PDB entry 3x3n coloured by chain, side view.
PDB entry 3x3n coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase.
Zhang XL, Li DF, Fleming J, Wang LW, Zhou Y, Wang DC, Zhang XE, Bi LJ
FASEB J 29 4804-14 (2015)
PMID: 26396239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ESX-1 secretion system ATPase EccB1 Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 46.22 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNR7 (Residues: 72-480; Coverage: 85%)
Gene names: Rv3869, eccB1
Sequence domains: Type VII secretion system ESX-1, transport TM domain B
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 31.84Å b: 122.65Å c: 60.42Å
α: 90° β: 101.27° γ: 90°
R-values:
R R work R free
0.204 0.202 0.234
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Microbial Offense vs Host Defense: Who Controls the TB Granuloma?
Martinot AJ. (2018)
4 more

2 mentions without citation

Crystallographic observation of the movement of the membrane-distal domain of the T7SS core component EccB1 from Mycobacterium tuberculosis.
Xie et al. (2016)
1 more