› 3wz5

X-ray diffraction
3.5Å resolution

Structure of the periplasmic domain of DotI (crystal form II)

Released:
DOI: 10.2210/pdb3wz5/pdb
PDB entry 3wz5 coloured by chain, front view.
PDB entry 3wz5 coloured by chain, side view.
PDB entry 3wz5 coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion.
Kuroda T, Kubori T, Thanh Bui X, Hyakutake A, Uchida Y, Imada K, Nagai H
Sci Rep 5 10912 (2015)
PMID: 26039110

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV secretion protein DotI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 144 amino acids
Theoretical weight: 15.73 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O54626 (Residues: 73-212; Coverage: 66%)
Gene names: C3927_01535, DIZ50_13030, I8Z25_002407, NCTC12000_00583, dotI, icmL
Sequence domains: Type-IV b secretion system, inner-membrane complex component

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: I432
Unit cell:
a: 230.328Å b: 230.328Å c: 230.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Mosaic Type IV Secretion Systems.
Christie PJ. (2016)
4 more

1 mention without citation

Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion.
Kuroda et al. (2015)