› 3wxa

X-ray diffraction
2.36Å resolution

X-ray crystal structural analysis of the complex between ALG-2 and Sec31A peptide

Released:
DOI: 10.2210/pdb3wxa/pdb
PDB entry 3wxa coloured by chain, front view.
PDB entry 3wxa coloured by chain, side view.
PDB entry 3wxa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the complex between penta-EF-hand ALG-2 protein and Sec31A peptide reveals a novel target recognition mechanism of ALG-2.
Takahashi T, Kojima K, Zhang W, Sasaki K, Ito M, Suzuki H, Kawasaki M, Wakatsuki S, Takahara T, Shibata H, Maki M
Int J Mol Sci 16 3677-99 (2015)
PMID: 25667979

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130900 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Programmed cell death protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 20.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75340 (Residues: 20-191; Coverage: 90%)
Gene names: ALG2, PDCD6
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Protein transport protein Sec31A Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O94979 (Residues: 837-848; Coverage: 1%)
Gene names: HSPC275, HSPC334, KIAA0905, SEC31A, SEC31L1

Ligands and Environments

1 bound ligand:
Ligand ZN 10 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P64
Unit cell:
a: 81.973Å b: 81.973Å c: 103.232Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.225 0.263
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Adaptor functions of the Ca2+-binding protein ALG-2 in protein transport from the endoplasmic reticulum.
Shibata H. (2019)
16 other articles

3 mentions without citation

Multifaceted Roles of ALG-2 in Ca(2+)-Regulated Membrane Trafficking.
Maki et al. (2016)
2 more