› 3wrs

X-ray diffraction
1.58Å resolution

Minute virus of mice non-structural protein-1N-terminal nuclease domain reveals a unique Zn2+ coordination in the active site pocket and shows a novel mode of DNA recognition at the origin of replication

Released:
DOI: 10.2210/pdb3wrs/pdb
PDB entry 3wrs coloured by chain, front view.
PDB entry 3wrs coloured by chain, side view.
PDB entry 3wrs coloured by chain, top view.
Source organism: Murine minute virus strain MVM prototype
Primary publication:
Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding.
Tewary SK, Liang L, Lin Z, Lynn A, Cotmore SF, Tattersall P, Zhao H, Tang L
Virology 476 61-71 (2015)
PMID: 25528417

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Initiator protein NS1 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.34 KDa
Source organism: Murine minute virus strain MVM prototype
Expression system: Escherichia coli
UniProt:
  • Canonical: P03134 (Residues: 1-255; Coverage: 38%)
Gene name: NS1
Sequence domains: Parvovirus non-structural protein 1

Ligands and Environments

1 bound ligand:
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21212
Unit cell:
a: 57.11Å b: 120.95Å c: 41.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.218
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Parvovirus Family Conundrum: What Makes a Killer?
Kailasan et al. (2015)
4 more

1 mention without citation

Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding.
Tewary et al. (2015)