› 3wq7

X-ray diffraction
1.68Å resolution

New crystal form of the hyperthermophilic family 12 endo-cellulase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3wq7/pdb
PDB entry 3wq7 coloured by chain, front view.
PDB entry 3wq7 coloured by chain, side view.
PDB entry 3wq7 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
A new crystal form of a hyperthermophilic endocellulase.
Kataoka M, Ishikawa K
Acta Crystallogr F Struct Biol Commun 70 878-83 (2014)
PMID: 25005081

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 36.02 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V2T0 (Residues: 1-319; Coverage: 100%)
Gene name: eglA
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand GOL 16 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 134.729Å b: 62.568Å c: 86.284Å
α: 90° β: 95.08° γ: 90°
R-values:
R R work R free
0.183 0.181 0.217
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)

1 mention without citation

A new crystal form of a hyperthermophilic endocellulase.
Kataoka et al. (2014)