› 3wlx

X-ray diffraction
2.51Å resolution

Crystal structure of low-specificity L-threonine aldolase from Escherichia coli

Released:
DOI: 10.2210/pdb3wlx/pdb
PDB entry 3wlx coloured by chain, front view.
PDB entry 3wlx coloured by chain, side view.
PDB entry 3wlx coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Qin H-M, Imai FL, Miyakawa T, Kataoka M, Okai M, Hou F, Ohtsuka J, Nagata K, Shimizu S, Tanokura M

Function and Biology Details

Reaction catalysed: 
L-allo-threonine = glycine + acetaldehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low specificity L-threonine aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 36.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P75823 (Residues: 1-333; Coverage: 100%)
Gene names: JW0854, b0870, ltaE, ybjU
Sequence domains: Beta-eliminating lyase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PLG 2 x PLG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 75.73Å b: 100.34Å c: 174.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.232
Expression system: Escherichia coli

Quick links