› 3wlr

X-ray diffraction
2.21Å resolution

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 609 amino acids
Theoretical weight: 65.75 KDa
Source organism: Hordeum vulgare subsp. vulgare
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9XEI3 (Residues: 26-630; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 3 x NAG
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P43212
Unit cell:
a: 100.688Å b: 100.688Å c: 183.294Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.218
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Research progress and the biotechnological applications of multienzyme complex.
Jiang et al. (2021)
1 more

1 mention without citation

Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans.
Hrmova et al. (2023)