› 3wf4

X-ray diffraction
2.3Å resolution

Crystal structure of human beta-galactosidase mutant I51T in complex with 6S-NBI-DGJ

Released:
DOI: 10.2210/pdb3wf4/pdb
PDB entry 3wf4 coloured by chain, front view.
PDB entry 3wf4 coloured by chain, side view.
PDB entry 3wf4 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Suzuki H, Ohto U, Shimizu T

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 678 amino acids
Theoretical weight: 76.61 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P16278 (Residues: 24-677; Coverage: 100%)
Gene names: ELNR1, GLB1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NAG 16 x NAG
Ligand CL 4 x CL
Ligand 6DJ 4 x 6DJ
Ligand SO4 8 x SO4
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 95.026Å b: 116.175Å c: 140.68Å
α: 90° β: 92.29° γ: 90°
R-values:
R R work R free
0.182 0.179 0.24
Expression system: Komagataella pastoris

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