› 3w9p

X-ray diffraction
2.1Å resolution

Crystal structure of monomeric FraC (second crystal form)

Released:
DOI: 10.2210/pdb3w9p/pdb
PDB entry 3w9p coloured by chain, front view.
PDB entry 3w9p coloured by chain, side view.
PDB entry 3w9p coloured by chain, top view.
Source organism: Actinia fragacea
Primary publication:
Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.
Tanaka K, Caaveiro JM, Morante K, González-Mañas JM, Tsumoto K
Nat Commun 6 6337 (2015)
PMID: 25716479

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DELTA-actitoxin-Afr1a Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 19.88 KDa
Source organism: Actinia fragacea
Expression system: Escherichia coli
UniProt:
  • Canonical: B9W5G6 (Residues: 1-179; Coverage: 100%)
Sequence domains: Sea anemone cytotoxic protein
Structure domains: Cytolysin/lectin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 58.98Å b: 60.51Å c: 80.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.216
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
31 more

2 mentions without citation

Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.
Tanaka et al. (2015)
1 more