› 3w7r

X-ray diffraction
1.68Å resolution

Structure of Human dihydroorotate dehydrogenase in complex with mii-4-097

Released:
DOI: 10.2210/pdb3w7r/pdb
PDB entry 3w7r coloured by chain, front view.
PDB entry 3w7r coloured by chain, side view.
PDB entry 3w7r coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Inaoka DK, Iida M, Tabuchi T, Lee N, Hashimoto S, Matsuoka S, Kuranaga T, Shiba T, Sakamoto K, Suzuki S, Balogun EO, Nara T, Aoki T, Inoue M, Honma T, Tanaka A, Harada S, Kita K

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (quinone), mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 42.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02127 (Residues: 29-395; Coverage: 93%)
Gene name: DHODH
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
7 bound ligands:
Ligand CL 1 x CL
Ligand DDQ 6 x DDQ
Ligand W7A 1 x W7A
Ligand GOL 22 x GOL
Ligand ORO 1 x ORO
Ligand SO4 4 x SO4
Ligand ACT 7 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3221
Unit cell:
a: 90.449Å b: 90.449Å c: 123.063Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.142 0.14 0.172
Expression system: Escherichia coli

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