› 3vwq

X-ray diffraction
1.7Å resolution

6-aminohexanoate-dimer hydrolase S112A/G181D/R187A/H266N/D370Y mutant complexd with 6-aminohexanoate

Released:
DOI: 10.2210/pdb3vwq/pdb
PDB entry 3vwq coloured by chain, front view.
PDB entry 3vwq coloured by chain, side view.
PDB entry 3vwq coloured by chain, top view.
Source organism: Paenarthrobacter ureafaciens
Entry authors: Kawashima Y, Shibata N, Negoro S, Higuchi Y

Function and Biology Details

Reaction catalysed: 
(N-(6-aminohexanoyl))(n) + H(2)O = (N-(6-aminohexanoyl))(n-1) + 6-aminohexanoate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-aminohexanoate-dimer hydrolase Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.82 KDa
Source organism: Paenarthrobacter ureafaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07061 (Residues: 1-28, 181-208, 266-296; Coverage: 22%)
  • Canonical: P07062 (Residues: 29-180, 209-265, 297-392; Coverage: 78%)
Gene names: nylB, nylB'
Sequence domains: Beta-lactamase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACA 1 x ACA
Ligand GOL 5 x GOL
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3221
Unit cell:
a: 96.4Å b: 96.4Å c: 113.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.189 0.208
Expression system: Escherichia coli

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