› 3vue

X-ray diffraction
2.7Å resolution

Crystal Structure of Rice Granule bound Starch Synthase I Catalytic Domain

Released:
DOI: 10.2210/pdb3vue/pdb
PDB entry 3vue coloured by chain, front view.
PDB entry 3vue coloured by chain, side view.
PDB entry 3vue coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Interdomain disulfide bridge in the rice granule bound starch synthase I catalytic domain as elucidated by X-ray structure analysis.
Momma M, Fujimoto Z
Biosci Biotechnol Biochem 76 1591-5 (2012)
PMID: 22878205

Function and Biology Details

Reaction catalysed: 
NDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = NDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Granule-bound starch synthase 1, chloroplastic/amyloplastic Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 59.35 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0DEV5 (Residues: 83-609; Coverage: 87%)
Gene names: 134P10.7, LOC_Os06g04200, Os06g0133000, P0679C08.19, WAXY, WX, WX-B
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P432
Unit cell:
a: 152.904Å b: 152.904Å c: 152.904Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.267
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Formation of starch in plant cells.
Pfister et al. (2016)
2 more

4 mentions without citation

Potato starch synthases: Functions and relationships.
Nazarian-Firouzabadi et al. (2017)
3 more