› 3vpx

X-ray diffraction
2.55Å resolution

Crystal structure of leucine dehydrogenase from a psychrophilic bacterium Sporosarcina psychrophila.

Released:
DOI: 10.2210/pdb3vpx/pdb
PDB entry 3vpx coloured by chain, front view.
PDB entry 3vpx coloured by chain, side view.
PDB entry 3vpx coloured by chain, top view.
Source organism: Sporosarcina psychrophila
Primary publication:
A psychrophilic leucine dehydrogenase from Sporosarcina psychrophila: Purification, characterization, gene sequencing and crystal structure analysis
Zhao Y, Wakamatsu T, Doi K, Sakuraba H, Ohshima T
J Mol Catal B Enzym 83 65-72 (2012)

Function and Biology Details

Reaction catalysed: 
L-leucine + H(2)O + NAD(+) = 4-methyl-2-oxopentanoate + NH(3) + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 39.39 KDa
Source organism: Sporosarcina psychrophila
UniProt:
  • Canonical: I0IJU1 (Residues: 1-364; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I4
Unit cell:
a: 135.589Å b: 135.589Å c: 123.553Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.29

Quick links