Function and Biology Details
Reaction catalysed:
Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A(2)pm) in 7-(L-Ala-gamma-D-Glu)-meso-A(2)pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A(2)pm. It is required that the D-terminal amino and carboxy groups of meso-A(2)pm are unsubstituted.
Biochemical function:
Biological process:
- not assigned
Cellular component:
Sequence domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191295 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidoglycan amidase Tse1
Molecule details ›
Chains: A, B, C, D
Length: 174 amino acids
Theoretical weight: 18.63 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
Structure domains: endopeptidase domain like (from Nostoc punctiforme)
Length: 174 amino acids
Theoretical weight: 18.63 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9I2Q1 (Residues: 1-154; Coverage: 100%)
Structure domains: endopeptidase domain like (from Nostoc punctiforme)
Immune protein Tsi1
Molecule details ›
Chains: E, F, G, H
Length: 192 amino acids
Theoretical weight: 20.95 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
Sequence domains: Immune protein Tsi1
Structure domains: Lipocalin
Length: 192 amino acids
Theoretical weight: 20.95 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
- Canonical: Q9I2Q0 (Residues: 1-172; Coverage: 100%)
Sequence domains: Immune protein Tsi1
Structure domains: Lipocalin
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-17A
Spacegroup:
P3221
Expression system: Escherichia coli
