› 3vmt

X-ray diffraction
2.3Å resolution

Crystal structure of Staphylococcus aureus membrane-bound transglycosylase in complex with a Lipid II analog

Released:
DOI: 10.2210/pdb3vmt/pdb
PDB entry 3vmt coloured by chain, front view.
PDB entry 3vmt coloured by chain, side view.
PDB entry 3vmt coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Crystal structure of Staphylococcus aureus transglycosylase in complex with a lipid II analog and elucidation of peptidoglycan synthesis mechanism.
Huang CY, Shih HW, Lin LY, Tien YW, Cheng TJ, Cheng WC, Wong CH, Ma C
Proc Natl Acad Sci U S A 109 6496-501 (2012)
PMID: 22493270

Function and Biology Details

Reaction catalysed: 
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monofunctional glycosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 30.31 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99T05 (Residues: 28-269; Coverage: 90%)
Gene names: SAV1874, mgt
Sequence domains: Transglycosylase
Structure domains: Biosynthetic peptidoglycan transglycosylase-like

Ligands and Environments

2 bound ligands:
Ligand LHI 1 x LHI
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 66.493Å b: 67.429Å c: 152.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.242
Expression system: Escherichia coli

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Citations

5 review citations

Bacterial cell division proteins as antibiotic targets.
den Blaauwen et al. (2014)
4 more

8 mentions without citation

Bioactive oligosaccharide natural products.
McCranie et al. (2014)
7 more