Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
ATP + H(2)O = ADP + phosphate
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
Non-structural protein 4 (preferred)
PDBe Complex ID:
PDB-CPX-143131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 4
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.58 KDa
Source organism: Murine coronavirus inf-MHV-A59
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus replicase NSP4, C-terminal
Structure domains: Coronavirus nonstructural protein 4 C-terminus
Length: 94 amino acids
Theoretical weight: 10.58 KDa
Source organism: Murine coronavirus inf-MHV-A59
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P0C6X9 (Residues: 3245-3333; Coverage: 1%)
Sequence domains: Coronavirus replicase NSP4, C-terminal
Structure domains: Coronavirus nonstructural protein 4 C-terminus
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-17A
Spacegroup:
P41212
Expression system: Escherichia coli BL21(DE3)
