› 3v8f

X-ray diffraction
3.8Å resolution

Crystal structure of crosslinked GltPh V216C-M385C mutant

Released:
DOI: 10.2210/pdb3v8f/pdb
PDB entry 3v8f coloured by chain, front view.
PDB entry 3v8f coloured by chain, side view.
PDB entry 3v8f coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structure of an asymmetric trimer of a bacterial glutamate transporter homolog.
Verdon G, Boudker O
Nat Struct Mol Biol 19 355-7 (2012)
PMID: 22343718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 422 amino acids
Theoretical weight: 44.59 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-417; Coverage: 98%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

3 bound ligands:
Ligand ASP 3 x ASP
Ligand NA 6 x NA
Ligand HG 3 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 124.3Å b: 199.81Å c: 111.251Å
α: 90° β: 117.08° γ: 90°
R-values:
R R work R free
0.244 0.243 0.255
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
23 more

6 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
5 more