› 3v5u

X-ray diffraction
1.9Å resolution

Structure of Sodium/Calcium Exchanger from Methanocaldococcus jannaschii DSM 2661

Released:
DOI: 10.2210/pdb3v5u/pdb
PDB entry 3v5u coloured by chain, front view.
PDB entry 3v5u coloured by chain, side view.
PDB entry 3v5u coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structural insight into the ion-exchange mechanism of the sodium/calcium exchanger.
Liao J, Li H, Zeng W, Sauer DB, Belmares R, Jiang Y
Science 335 686-90 (2012)
PMID: 22323814

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized membrane protein MJ0091 Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.24 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57556 (Residues: 1-302; Coverage: 100%)
Gene name: MJ0091
Sequence domains: Sodium/calcium exchanger protein
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand NA 3 x NA
Ligand CA 2 x CA
Ligand ACT 4 x ACT
Ligand OLC 5 x OLC
Ligand MYS 9 x MYS
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 49.49Å b: 72.879Å c: 96.206Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.21
Expression system: Escherichia coli

Quick links

Citations

37 review citations

The ABCs of membrane transporters in health and disease (SLC series): introduction.
Hediger et al. (2013)
36 more

27 mentions without citation

Influences of membrane mimetic environments on membrane protein structures.
Zhou et al. (2013)
26 more