› 3uu3

X-ray diffraction
3.15Å resolution

The GLIC pentameric Ligand-Gated Ion Channel Loop2-20' oxidized mutant in a locally-closed conformation (LC1 subtype)

Released:
DOI: 10.2210/pdb3uu3/pdb
PDB entry 3uu3 coloured by chain, front view.
PDB entry 3uu3 coloured by chain, side view.
PDB entry 3uu3 coloured by chain, top view.
Source organism: Gloeobacter violaceus
Primary publication:
A locally closed conformation of a bacterial pentameric proton-gated ion channel.
Prevost MS, Sauguet L, Nury H, Van Renterghem C, Huon C, Poitevin F, Baaden M, Delarue M, Corringer PJ
Nat Struct Mol Biol 19 642-9 (2012)
PMID: 22580559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 321 amino acids
Theoretical weight: 36.54 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 44-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 181.624Å b: 134.234Å c: 160.046Å
α: 90° β: 102.47° γ: 90°
R-values:
R R work R free
0.25 0.248 0.272
Expression system: Escherichia coli

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Citations

21 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
20 more

3 mentions without citation

Correlating structural and energetic changes in glycine receptor activation.
Scott et al. (2015)
2 more