› 3us2

X-ray diffraction
4.2Å resolution

Structure of p63 DNA Binding Domain in Complex with a 19 Base Pair A/T Rich Response Element Containing Two Half Sites with a Single Base Pair Overlap

Released:
DOI: 10.2210/pdb3us2/pdb
PDB entry 3us2 coloured by chain, front view.
PDB entry 3us2 coloured by chain, side view.
PDB entry 3us2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pliable DNA conformation of response elements bound to transcription factor p63.
Chen C, Gorlatova N, Herzberg O
J Biol Chem 287 7477-86 (2012)
PMID: 22247550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117005 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Tumor protein 63 Chains: A, B, C, D, G, H, I, J
Molecule details ›
Chains: A, B, C, D, G, H, I, J
Length: 203 amino acids
Theoretical weight: 22.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H3D4 (Residues: 166-362; Coverage: 29%)
Gene names: KET, P63, P73H, P73L, TP63, TP73L
Sequence domains: P53 DNA-binding domain
5'-D(*AP*AP*AP*CP*AP*TP*GP*TP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*T)-3' Chains: E, K
Molecule details ›
Chains: E, K
Length: 19 nucleotides
Theoretical weight: 5.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*AP*CP*AP*TP*GP*TP*TP*T)-3' Chains: F, L
Molecule details ›
Chains: F, L
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 119.739Å b: 180.544Å c: 98.174Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.34 0.326 0.334
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Roles of p53 Family Structure and Function in Non-Canonical Response Element Binding and Activation.
Cai et al. (2019)
1 more

5 mentions without citation

Disease-associated mutations disrupt functionally important regions of intrinsic protein disorder.
Vacic et al. (2012)
4 more