› 3ung

X-ray diffraction
2.31Å resolution

Structure of the Cmr2 subunit of the CRISPR RNA silencing complex

Released:
DOI: 10.2210/pdb3ung/pdb
PDB entry 3ung coloured by chain, front view.
PDB entry 3ung coloured by chain, side view.
PDB entry 3ung coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structure of the Cmr2 subunit of the CRISPR-Cas RNA silencing complex.
Cocozaki AI, Ramia NF, Shao Y, Hale CR, Terns RM, Terns MP, Li H
Structure 20 545-53 (2012)
PMID: 22405013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 73.167Å b: 85.07Å c: 140.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
14 more

4 mentions without citation

Annotation and Classification of CRISPR-Cas Systems.
Makarova et al. (2015)
3 more