› 3umg

X-ray diffraction
2.25Å resolution

Crystal Structure of the Defluorinating L-2-Haloacid Dehalogenase Rha0230

Released:
DOI: 10.2210/pdb3umg/pdb
PDB entry 3umg coloured by chain, front view.
PDB entry 3umg coloured by chain, side view.
PDB entry 3umg coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Chan PWY, Savchenko A, Yakunin AF, Edwards EA, Pai EF

Function and Biology Details

Reaction catalysed: 
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloacid dehalogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 254 amino acids
Theoretical weight: 27.56 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0SK70 (Residues: 1-254; Coverage: 100%)
Gene name: RHA1_ro00230
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 7 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 102.18Å b: 148.65Å c: 152.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links