› 3ukg

X-ray diffraction
2.95Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116992 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein RAP1 Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 28.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P11938 (Residues: 360-601; Coverage: 29%)
Gene names: GRF1, N1310, RAP1, TUF1, YNL216W
Sequence domains:
Structure domains: Homeodomain-like
telomeric DNA Chain: C
Molecule details ›
Chain: C
Length: 31 nucleotides
Theoretical weight: 9.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
telomeric DNA Chain: D
Molecule details ›
Chain: D
Length: 31 nucleotides
Theoretical weight: 9.67 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 40.595Å b: 102.944Å c: 116.755Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.191 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 citation in other articles

ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo.
Zentner et al. (2015)
13 more

8 mentions without citation

Shepherding DNA ends: Rif1 protects telomeres and chromosome breaks.
Fontana et al. (2018)
7 more