› 3ug0

X-ray diffraction
2.09Å resolution

Crystal structure of a Trp-less green fluorescent protein translated by the simplified genetic code

Released:
DOI: 10.2210/pdb3ug0/pdb
PDB entry 3ug0 coloured by chain, front view.
PDB entry 3ug0 coloured by chain, side view.
PDB entry 3ug0 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Simplification of the genetic code: restricted diversity of genetically encoded amino acids.
Kawahara-Kobayashi A, Masuda A, Araiso Y, Sakai Y, Kohda A, Uchiyama M, Asami S, Matsuda T, Ishitani R, Dohmae N, Yokoyama S, Kigawa T, Nureki O, Kiga D
Nucleic Acids Res 40 10576-84 (2012)
PMID: 22909996

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.63 KDa
Source organism: Aequorea victoria
Expression system: CELL-FREE SYNTHESIS
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 51.784Å b: 63.05Å c: 67.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.241
Expression system: CELL-FREE SYNTHESIS

Quick links

Citations

1 review citation

Overcoming Challenges in Engineering the Genetic Code.
Lajoie et al. (2016)
6 other articles

1 mention without citation

Simplification of the genetic code: restricted diversity of genetically encoded amino acids.
Kawahara-Kobayashi et al. (2012)