› 3uf2

X-ray diffraction
2.75Å resolution

Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine

Released:
DOI: 10.2210/pdb3uf2/pdb
PDB entry 3uf2 coloured by chain, front view.
PDB entry 3uf2 coloured by chain, side view.
PDB entry 3uf2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric competitive inactivation of hematopoietic CSF-1 signaling by the viral decoy receptor BARF1.
Elegheert J, Bracke N, Pouliot P, Gutsche I, Shkumatov AV, Tarbouriech N, Verstraete K, Bekaert A, Burmeister WP, Svergun DI, Lambrecht BN, Vergauwen B, Savvides SN
Nat Struct Mol Biol 19 938-47 (2012)
PMID: 22902366

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage colony-stimulating factor 1 43 kDa subunit Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 153 amino acids
Theoretical weight: 17.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09603 (Residues: 33-181; Coverage: 29%)
Gene name: CSF1
Sequence domains: Macrophage colony stimulating factor-1 (CSF-1)
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 61.15Å b: 104.49Å c: 116.24Å
α: 90° β: 105.29° γ: 90°
R-values:
R R work R free
0.142 0.137 0.19
Expression system: Escherichia coli

Quick links

Citations

13 review citations

CSF-1 receptor signaling in myeloid cells.
Stanley et al. (2014)
12 more

4 mentions without citation

Challenging the state of the art in protein structure prediction: Highlights of experimental target structures for the 10th Critical Assessment of Techniques for Protein Structure Prediction Experiment CASP10.
Kryshtafovych et al. (2014)
3 more