› 3u6y

X-ray diffraction
2Å resolution

Crystal structure of ALBA2-DNA complex

Released:
DOI: 10.2210/pdb3u6y/pdb
PDB entry 3u6y coloured by chain, front view.
PDB entry 3u6y coloured by chain, side view.
PDB entry 3u6y coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of archaeal chromatin protein Alba2-double-stranded DNA complex from Aeropyrum pernix K1.
Tanaka T, Padavattan S, Kumarevel T
J Biol Chem 287 10394-10402 (2012)
PMID: 22334696

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116972 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA/RNA-binding protein Alba 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 102 amino acids
Theoretical weight: 11.4 KDa
Source organism: Aeropyrum pernix K1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YAX2 (Residues: 1-102; Coverage: 100%)
Gene names: APE_1823, albA2, ape10b2
Sequence domains: Alba
Structure domains: Alba-like domain
DNA (5'-D(*CP*CP*GP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.48 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*GP*CP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 5 nucleotides
Theoretical weight: 1.52 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 33.556Å b: 74.29Å c: 88.198Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

The interplay between nucleoid organization and transcription in archaeal genomes.
Peeters et al. (2015)
6 more

7 mentions without citation

Archaea: The Final Frontier of Chromatin.
Laursen et al. (2021)
6 more