› 3u39

X-ray diffraction
2.79Å resolution

Crystal Structure of the apo Bacillus Stearothermophilus phosphofructokinase

Released:
DOI: 10.2210/pdb3u39/pdb
PDB entry 3u39 coloured by chain, front view.
PDB entry 3u39 coloured by chain, side view.
PDB entry 3u39 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of the apo form of Bacillus stearothermophilus phosphofructokinase.
Mosser R, Reddy MC, Bruning JB, Sacchettini JC, Reinhart GD
Biochemistry 51 769-75 (2012)
PMID: 22212099

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 34.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00512 (Residues: 1-319; Coverage: 100%)
Gene names: pfk, pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 5 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 201.909Å b: 113.383Å c: 76.627Å
α: 90° β: 104.03° γ: 90°
R-values:
R R work R free
0.202 0.199 0.26
Expression system: Escherichia coli BL21(DE3)

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Citations

6 citation in other articles

Allosteric regulation in phosphofructokinase from the extreme thermophile Thermus thermophilus.
McGresham et al. (2014)
5 more

1 mention without citation

Structure of the apo form of Bacillus stearothermophilus phosphofructokinase.
Mosser et al. (2012)