3twi

X-ray diffraction
2.55Å resolution

Variable Lymphocyte Receptor Recognition of the Immunodominant Glycoprotein of Bacillus anthracis Spores

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-109100 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BclA C-terminal domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 160 amino acids
Theoretical weight: 16.42 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81JD7 (Residues: 245-382; Coverage: 36%)
Gene names: GBAA_1222, bclA
Sequence domains: BclA C-terminal domain
Structure domains: Jelly Rolls
LRRNT domain-containing protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 179 amino acids
Theoretical weight: 19.47 KDa
Source organism: Petromyzon marinus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A9Z0I5 (Residues: 21-188; Coverage: 67%)
Gene name: VLR-B
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 74.809Å b: 146.528Å c: 209.501Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda