› 3trc

X-ray diffraction
1.65Å resolution

Structure of the GAF domain from a phosphoenolpyruvate-protein phosphotransferase (ptsP) from Coxiella burnetii

Released:
DOI: 10.2210/pdb3trc/pdb
PDB entry 3trc coloured by chain, front view.
PDB entry 3trc coloured by chain, side view.
PDB entry 3trc coloured by chain, top view.
Source organism: Coxiella burnetii
Primary publication:
Structural genomics for drug design against the pathogen Coxiella burnetii.
Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD
Proteins 83 2124-36 (2015)
PMID: 26033498

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoenolpyruvate--protein phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 18.93 KDa
Source organism: Coxiella burnetii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83BF9 (Residues: 8-175; Coverage: 22%)
Gene names: CBU_1550, ptsP
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 54.869Å b: 54.869Å c: 105.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.
Martins et al. (2015)
3 more

2 mentions without citation

Single expressed glycine receptor domains reconstitute functional ion channels without subunit-specific desensitization behavior.
Meiselbach et al. (2014)
1 more