› 3tka

X-ray diffraction
2.25Å resolution

crystal structure and solution saxs of methyltransferase rsmh from E.coli

Released:
DOI: 10.2210/pdb3tka/pdb
PDB entry 3tka coloured by chain, front view.
PDB entry 3tka coloured by chain, side view.
PDB entry 3tka coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal and solution structures of methyltransferase RsmH provide basis for methylation of C1402 in 16S rRNA.
Wei Y, Zhang H, Gao ZQ, Wang WJ, Shtykova EV, Xu JH, Liu QS, Dong YH
J Struct Biol 179 29-40 (2012)
PMID: 22561317

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase H Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 38.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P60390 (Residues: 1-313; Coverage: 100%)
Gene names: JW0080, b0082, mraW, rsmH, yabC
Sequence domains: MraW methylase family
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
3 bound ligands:
Ligand CTN 1 x CTN
Ligand SO4 5 x SO4
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 1W2B
Spacegroup: I41
Unit cell:
a: 85.66Å b: 85.66Å c: 107.059Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis.
Salaikumaran et al. (2022)
12 other articles

2 mentions without citation

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.
Van Haute et al. (2019)
1 more