› 3tij

X-ray diffraction
2.44Å resolution

Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae

Released:
DOI: 10.2210/pdb3tij/pdb
PDB entry 3tij coloured by chain, front view.
PDB entry 3tij coloured by chain, side view.
PDB entry 3tij coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae at 2.4 Å.
Johnson ZL, Cheong CG, Lee SY
Nature 483 489-93 (2012)
PMID: 22407322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside permease Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 44.17 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KPL5 (Residues: 1-418; Coverage: 100%)
Gene name: VC_2352
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand URI 1 x URI
Ligand DMU 5 x DMU
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P63
Unit cell:
a: 119.717Å b: 119.717Å c: 83.136Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.228
Expression system: Escherichia coli

Quick links

Citations

14 review citations

SLC transporters as therapeutic targets: emerging opportunities.
Lin et al. (2015)
13 more

14 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
13 more