› 3tfe

X-ray diffraction
1.99Å resolution

Crystal structure of an H-NOX protein from Nostoc sp. PCC 7120, L66W mutant under 6 atm of xenon

Released:
DOI: 10.2210/pdb3tfe/pdb
PDB entry 3tfe coloured by chain, front view.
PDB entry 3tfe coloured by chain, side view.
PDB entry 3tfe coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Tunnels modulate ligand flux in a heme nitric oxide/oxygen binding (H-NOX) domain.
Winter MB, Herzik MA, Kuriyan J, Marletta MA
Proc Natl Acad Sci U S A 108 E881-9 (2011)
PMID: 21997213

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.29 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-189; Coverage: 100%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand XE 4 x XE
Ligand MLA 3 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P213
Unit cell:
a: 123.295Å b: 123.295Å c: 123.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.173
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structure and regulation of soluble guanylate cyclase.
Derbyshire et al. (2012)
11 more

1 mention without citation

Tunnels modulate ligand flux in a heme nitric oxide/oxygen binding (H-NOX) domain.
Winter et al. (2011)